Fig. 3. Podocin and desmin staining in podocytes with or without visfatin and/or Asc siRNA transfection or WEHD treatment. Cultured podocytes were treated with or without visfatin for 16 h. A: Typical fluorescent microscopic images of podocin (upper images) and desmin (lower images) in podocytes with or without visfatin and/or Asc siRNA transfection or WEHD treatment (n=5/group, Original magnification, ×400). C: Values are arithmetic means ± SE (n=5 each group) of summarized data showing the mRNA expression of podocin. Ctrl: Control, Vehi: Vehicle, Scram: Scramble, Asc si: Asc siRNA, * Significant difference (P<0.05) compared to the control group; ^# Significant difference (P<0.05) compared to the visfatin (vehicle) group.